Applied Sciences, Vol. 14, Pages 11753: Molecular Insights into the Interactions Between Human Serum Albumin and Phospholipid Membranes

Applied Sciences, Vol. 14, Pages 11753: Molecular Insights into the Interactions Between Human Serum Albumin and Phospholipid Membranes

Applied Sciences doi: 10.3390/app142411753

Authors: Maciej Przybyłek Piotr Bełdowski Damian Ledziński Zbigniew Lutowski Adam Mazurkiewicz Przemysław Raczyński Andra Dedinaite Per M. Claesson

In this study, molecular dynamics simulations were employed to analyze interactions between phospholipid membranes and human serum albumin (HSA) in the presence of mono- and divalent cations. Two types of membranes, composed of dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidylethanolamine (DPPE), were utilized. The results revealed that both systems exhibited high stability. The DPPE complexes displayed a greater affinity for albumin compared to DPPC. The high stability of the complexes was attributed to a high number of ionic contacts and hydrogen bonds. The presence of mono- and divalent metal cations significantly influenced the membrane’s capacity to bind proteins. However, these effects varied depending on the phospholipid composition of the bilayer. The studies confirmed the relatively low ability of DPPC to bind potassium ions, as previously observed by others. Consequently, the DPPC/HSA/K+ complex was found to be the least stable among the systems studied. While DPPC interactions were limited to HSA domains I and II, DPPE was able to interact with all domains of the protein. Both lipid bilayers exhibited substantial structural changes and characteristic curvature induced by interactions with HSA, which confirms the formation of relatively strong interactions capable of influencing the arrangement of the phospholipids.