Cancers, Vol. 15, Pages 1714: Computational Analysis and Experimental Testing of the Molecular Mode of Action of Gatastatin and Its Derivatives

Cancers, Vol. 15, Pages 1714: Computational Analysis and Experimental Testing of the Molecular Mode of Action of Gatastatin and Its Derivatives

Cancers doi: 10.3390/cancers15061714

Authors: Paola Vottero Qian Wang Marek Michalak Maral Aminpour Jack Adam Tuszynski

Given its critical role in cell mitosis, the tubulin γ chain represents a viable chemotherapeutic target to solve the specificity issues associated with targeting α and β tubulin. Since γ tubulin is overexpressed in glioblastoma multiforme (GBM) and some breast lesions, the glaziovianin A derivative gatastatin, presented as a γ-tubulin-specific inhibitor, could yield a successful therapeutic strategy. The present work aims to identify the binding sites and modes of gatastatin and its derivatives through molecular-docking simulations. Computational binding free energy predictions were compared to experimental microscale thermophoresis assay results. The computational simulations did not reveal a strong preference toward γ tubulin, suggesting that further derivatization may be needed to increase its specificity.